Dopaminergic neuronal differentiation from rat embryonic neural precursors by Nurr1 overexpression
نویسندگان
چکیده
منابع مشابه
Role of Nurr1 and Ret in inducing rat embryonic neural precursors to dopaminergic neurons.
BACKGROUND Nurr1, a member of the nuclear receptor superfamily of transcription factors, is highly expressed in midbrain dopaminergic (DA) neurons. Ret is a member of the receptor tyrosine kinase (RTK) superfamily and a critical signal transducing subunit of receptors for glial cell line-derived neurotrophic factor (GDNF). Both Nurr1 and Ret play important roles in the development of DA neurons...
متن کاملTemporally induced Nurr1 can induce a non-neuronal dopaminergic cell type in embryonic stem cell differentiation.
The nuclear transcription factor Nurr1 is involved in the development and maintenance of the midbrain dopaminergic (DA) neuronal phenotype. We analysed the cellular and biological effects of Nurr1 during embryonic stem (ES) cell differentiation using the ROSA26-engineered Tet-inducible ES cell line J1-rtTA that does not express transgenes in mature neurons. Induction of Nurr1 at nestin-positive...
متن کاملGenetic engineering of mouse embryonic stem cells by Nurr1 enhances differentiation and maturation into dopaminergic neurons.
Nurr1 is a transcription factor critical for the development of midbrain dopaminergic (DA) neurons. This study modified mouse embryonic stem (ES) cells to constitutively express Nurr1 under the elongation factor-1alpha promoter. The Nurr1-expression in ES cells lead to up-regulation of all DA neuronal markers tested, resulting in about a 4- to 5-fold increase in the proportion of DA neurons. In...
متن کاملRestriction of Neural Precursor Ability to Respond to Nurr1 by Early Regional Specification
During neural development, spatially regulated expression of specific transcription factors is crucial for central nervous system (CNS) regionalization, generation of neural precursors (NPs) and subsequent differentiation of specific cell types within defined regions. A critical role in dopaminergic differentiation in the midbrain (MB) has been assigned to the transcription factor Nurr1. Nurr1 ...
متن کاملAcquisition of in vitro and in vivo functionality of Nurr1-induced dopamine neurons.
Neural precursor cells provide an expandable source of neurons and glia for basic and translational applications. However, little progress has been made in directing naive neural precursors toward specific neuronal fates such as midbrain dopamine (DA) neurons. We have recently demonstrated that transgenic expression of the nuclear orphan receptor Nurr1 is sufficient to drive dopaminergic differ...
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ژورنال
عنوان ژورنال: Journal of Neurochemistry
سال: 2003
ISSN: 0022-3042,1471-4159
DOI: 10.1046/j.1471-4159.2003.01780.x